The balance between cell survival and death is fine-tuned by (non-) proteolytic ubiquitination of key proteins, among them components of pro-survival signaling, like NF-kB and cell death, such as extrinsic and intrinsic apoptosis and necroptosis. Apart from classical biochemistry, we use state-of-the-art methodologies, such as high-content and super-resolution imaging, CRISPR/Cas9 and ubiquitin biochemistry to understand the regulation of ubiquitination in cell fate control in tumor cell lines and spheroids, mouse and patient-derived primary tumor organoids and preclininal research models.
Our current work aims to further develop and apply novel tools to study ubiquitination in cellular processes, like autophagy and cell survival/death.